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KMID : 0620920070390040469
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Experimental & Molecular Medicine
2007 Volume.39 No. 4 p.469 ~ p.476
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Cyclooxygenase-2 promotes cell proliferation, migration and invasion in U2OS human osteosarcoma cells
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Lee Eun-Jeong
Choi Eun-Mi
Kim So-Ra
Park Jung-Hea
Kim Hyun-Sook
Ha Kwon-Soo
Kim Young-Myeong
Kim Sung-Soo
Choe Myeon
Kim Jong-Il
Han Jeong-A
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Abstract
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Osteosarcoma is the most common primary bone tumor, but the pathogenesis is not well understood. While cyclooxygeanse-2 (COX-2) is known to be closely associated with tumor growth and metastasis in several kinds of human tumors, the function of COX-2 in osteosarcoma is unclear. Therefore, to investigate the function of COX-2 in osteosarcoma, we established stable cell lines overexpressing COX-2 in U2OS human osteosarcoma cells. COX-2 overexpression as well as prostaglandin E2 treatment promoted proliferation of U2OS cells. In addition, COX-2 overexpression enhanced mobility and invasiveness of U2OS cells, which was accompanied by increases of matrix metalloproteinase- 2 and -9 (MMP-2 and -9) activities. Selective COX-2 inhibitors, NS-398 and celecoxib, inhibited cell proliferation and abrogated the enhanced mobility, invasiveness and MMP activities induced by COX-2 overexpression. These results suggest that COX-2 is directly associated with cell proliferation, migration and invasion in human osteosarcoma cells, and the therapeutic value of COX-2 inhibitors should be evaluated continuously.
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KEYWORD
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cell movement, cell proliferation, cyclooxygenase-matrix metalloproteinases, osteosarcoma
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